Age-Related Changes in Blood Plasma Alanine aminotransferase in Partridges (Alectoris Chukar) With the Complex Use of Vitamins and an Antistress Drug

The article presents the results of the influence of vitamins and an anti-stress drug on the biochemical parameters of partridge blood.

In order to identify the dynamics of age-related changes in the activity of alanine aminotransferase in the blood of partridges Alectoris chukar with the complex use of vitamins and an anti-stress drug, we conducted studies on 30 partridges during the period of life from 1, 7, 10, 20, 30, 60 to 90 days.

Determination of the activity of the enzyme alanine amino transferase in the blood of partridges was carried out on a Specol 1500 spectrophotometer (Analitik Jena) according to the method of R Rej., M Hoder, U.H Bergmeyer at a wavelength of 540 nm [25]. 

The object of research was the venous blood of partridges. After the inclusion in the daily diet of partridges of a complex of vitamins (A-20000 IU, D3 - 1250 IU, E - 50 mg) and an anti-stress drug (succinic acid) at a dose of 0.05 g per 1 kg of body weight per day, a tendency to improve biochemical indicators of the activity of the enzyme alanyl aminotransferase. Experimentally revealed a decrease in the activity of the enzyme alanyl aminotransferase in the blood of partridges, which were 1.09, 1.17, 1.13, 1.47, 1.44, 1.37 times greater than the indicators of the latter in the control groups of partridges. The activity value of the alanyl aminotransferase enzyme ranged from 0.02 to 0.06 U/ml, with the maximum value reached at 30 days of age.

Data on the effect of a complex of vitamins and anti-stress drugs on the biochemical parameters of partridge blood was not found in the scientific literature, so the aim of the work was to find out the effect of a complex of vitamins and an anti-stress drug on age-related changes in the activity of the blood plasma alanine aminotransferase enzyme in partridges. The literature provides data on the role of enzyme systems in the regulation of growth and development, the formation of the productive qualities of poultry, including the content of aspartate aminotransferase and alanine aminotransferase in hepatocytes [1, 3]. The work of A. V. Antipov and co-authors [4] presents the results of a study of the effect of a probiotic feed additive on the body and quality indicators of quail meat.

According to research results, the recommended rate of a bioproduct in the diet is 2

The control and experimental groups of pheasants were selected according to the principle of analogue groups. During the experimental period, the following was taken into account: the safety of the livestock - by daily detection of dead birds with the establishment of the causes of death, the cost of feed per unit of production. All experiments were repeated and ended with a production check. Studies were carried out in the blood of 60 pheasants in the postembryonic period at the age of 1 to 60 days of age. Determination of the activity of the enzyme alanine aminotransferase in the blood of partridges was carried out on a Specol 1500 spectrophotometer (Analitik Jena) according to the method of R Rej., M Hoder, U.H Bergmeyer at a wavelength of 540 nm [25]. The significance of differences between groups was assessed taking into account Student's t-test in accordance with the generally accepted methodology [26].

The studies were carried out on 60 partridges from 1-90 days of age. The birds were divided into 4 groups of 5 animals in each group. The birds were kept in the vivarium and received the main diet, represented by factory-made compound feed with free access to drinking water. A complex of vitamins (A-20000 IU, D3 - 1250 IU, E - 50 mg) and an anti-stress drug (succinic acid) at a dose of 0.01, 0.03, 0.05 g per 1 kg of body weight per day were added to the diet of the experimental groups of partridges. The preparations were used with food for 90 days according to the experimental scheme presented in Table. 1.

Table 1: Scheme of experiments on pheasants.

Тable 2 shows the dynamics of the population of partridges of the control and experienced groups. From the data presented in Table 2, it can be seen that when a vitamin complex and an anti-stress drug are added to the diet of the experimental groups of partridges, there is a slight fluctuation in the number of both the control groups of partridges and the experimental groups.

From the obtained experimental data, it can be seen that after adding to the diet of birds a complex of vitamins (A-20000 IU, D3 - 1250 IU, E - 50 mg) and an anti-stress drug (succinic acid) at a dose of 0.01, 0.03, 0.05 g per 1 kg body weight per day, in all experimental groups there was a tendency to increase the survival of partridges from 85.0% to 98.3%. An improvement in biochemical parameters, including the activity of the alanyl amino-transferase enzyme, was experimentally revealed. Revealed a decrease in the activity of the enzyme alanyl amino transferase 1.09, 1.17, 1.13, 1.47, 1.44, 1.37 times compared with the indicators of the control groups of partridges (table 1). Thus, it must be stated that the data obtained from experimental studies indicate that when a complex of vitamins (A-20000 IU, D3 - 1250 IU, E - 50 mg) and an anti-stress drug (succinic acid 0.05 g) are added to the diet of birds per 1 kg of body weight per day), their positive effect is noted both on the productivity of pheasants and on the enzymatic activity of alanyl aminotransferase, and at the same time, the selected doses of vitamins and an antistress drug are optimal.

Figure 1: The activity of the enzyme alanyl aminotransferase in the blood of partridges.

The above comparative analysis of biochemical parameters indicates a positive effect of a complex of vitamins and an anti-stress drug on the functional activity of the liver. The activity of the alanyl aminotransferase enzyme in the blood of partridges indicates the destruction of tissue cells containing them and an increased release of this enzyme into the blood. An increase in the activity of alanyl aminotransferase in the blood of partridges is possible before the clinical manifestation of the pathological process. It should be noted that the content of the enzyme in the liver and the rate of their release from cells into the blood most affect the activity of the enzyme in the blood of partridges, which also affects their survival. Thus, based on the above, according to a comparative analysis of the biochemical parameters of the blood serum of partridges, it can be stated that when a complex of vitamins (A-20000 IU, D3 - 1250 IU, E - 50 mg) and an anti-stress drug (succinic acid 0, 05 g per 1 kg of body weight per day), the latter have a positive effect on survival and on the metabolic rate in all experimental groups of partridges.

• Revealed a decrease in the activity of the enzyme alanyl aminotransferase 1.09, 1.17, 1.13, 1.47, 1.44, 1.37 times compared with the indicators of the control groups of partridges
• The level of alanyl aminotransferase against the background of the use of a complex of vitamins (A-20000 IU, D3 - 1250 IU, E - 50 mg) and the anti-stress preparation of succinic acid at a dose of 0.05 g per 1 kg of body weight per day decreased by an average of 0.01 u/l in each test group, compared with control groups.